Anticoagulant and Fibrinogenolytic Properties of Seed Extract of Psidium guajava

نویسندگان

چکیده

The objective of this study was to identify and evaluate for the first time anticoagulant fibrinogenolytic protease from Psidium guajava seed extract. extract in sodium dodecyl sulfate polyacrylamide gel electrophoresis showed protein bands high molecular weight region between 122 kDa 47 two distinct lower at ~16.6 ~14.3 kDa. content found be 1 mg/ml with specific activity 0.8 U. proteolytic supported by zymography studies using casein/gelatin as substrates. hydrolysed all subunits fibrinogen alpha-polymer alpha-chain fibrin a concentration 20 μg 120 μg, respectively. prolonged clotting recalcification control 161 s 487 s, increasing ~3 folds. This indicates nature associated Our provides time, scientific validation presence property

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ژورنال

عنوان ژورنال: Indian Journal of Pharmaceutical Sciences

سال: 2021

ISSN: ['0250-474X', '1998-3743']

DOI: https://doi.org/10.36468/pharmaceutical-sciences.843